Project

Part:BBa_I717000:Design

Designed by: Blair Lyons   Group: iGEM07_Virginia_Tech   (2007-10-03)


acGFP<-Pr(lambda)->dsRED


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1544
    Illegal PstI site found at 359
    Illegal PstI site found at 560
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1545
    Illegal PstI site found at 359
    Illegal PstI site found at 560
    Illegal PstI site found at 1559
    Illegal NotI site found at 7
    Illegal NotI site found at 1552
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 217
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1545
    Illegal PstI site found at 359
    Illegal PstI site found at 560
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1545
    Illegal PstI site found at 359
    Illegal PstI site found at 560
    Illegal PstI site found at 1559
    Illegal NgoMIV site found at 275
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During design we had to account for differing strengths of RBS in either fluorescent protein gene, since we wanted both genes to be expressed equally. We also had to modify the bistable switch from lambda so that it was less cumbersome.


Source

AcGFP and dsRED are commercial genes with commercial sequences. The lambda sequence was obtained from literature.

References